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Effect of OBNC microspheres on regulating MSCs. A) The expression of FAK and p-FAK detected by Western blot analysis. B) Quantitative analysis of the Western blot results. C) The expression of the integrin-α5 gene. D) The expression of the integrin-α8 gene. E) The expression of the Lamb-2 gene. F) The expression of the Spp-1 gene. G) The expression of the Vav-3 gene. H) The expression of the CDC-42 gene. I) Detection of the CDC-42 active protein by a <t>GLISA</t> kit. J) Detection of Rac-1 active protein by a GLISA kit. K) Detection of Rho A active protein by a GLISA kit. L) Mechanism of OBNC hydrogels triggering the membrane receptor switch and activating integrin receptors. (ns: non-significant, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001).
Rhoa Glisa Activation Assay, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of OBNC microspheres on regulating MSCs. A) The expression of FAK and p-FAK detected by Western blot analysis. B) Quantitative analysis of the Western blot results. C) The expression of the integrin-α5 gene. D) The expression of the integrin-α8 gene. E) The expression of the Lamb-2 gene. F) The expression of the Spp-1 gene. G) The expression of the Vav-3 gene. H) The expression of the CDC-42 gene. I) Detection of the CDC-42 active protein by a <t>GLISA</t> kit. J) Detection of Rac-1 active protein by a GLISA kit. K) Detection of Rho A active protein by a GLISA kit. L) Mechanism of OBNC hydrogels triggering the membrane receptor switch and activating integrin receptors. (ns: non-significant, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001).
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rac1 glisa activation assay  (Cytoskeleton Inc)
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Effect of OBNC microspheres on regulating MSCs. A) The expression of FAK and p-FAK detected by Western blot analysis. B) Quantitative analysis of the Western blot results. C) The expression of the integrin-α5 gene. D) The expression of the integrin-α8 gene. E) The expression of the Lamb-2 gene. F) The expression of the Spp-1 gene. G) The expression of the Vav-3 gene. H) The expression of the CDC-42 gene. I) Detection of the CDC-42 active protein by a <t>GLISA</t> kit. J) Detection of Rac-1 active protein by a GLISA kit. K) Detection of Rho A active protein by a GLISA kit. L) Mechanism of OBNC hydrogels triggering the membrane receptor switch and activating integrin receptors. (ns: non-significant, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001).
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hASCs' relative <t>metabolic</t> <t>activity</t> in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining <t>cells'</t> nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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hASCs' relative <t>metabolic</t> <t>activity</t> in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining <t>cells'</t> nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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hASCs' relative <t>metabolic</t> <t>activity</t> in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining <t>cells'</t> nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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hASCs' relative <t>metabolic</t> <t>activity</t> in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining <t>cells'</t> nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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hASCs' relative <t>metabolic</t> <t>activity</t> in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining <t>cells'</t> nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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hASCs' relative <t>metabolic</t> <t>activity</t> in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining <t>cells'</t> nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Effect of OBNC microspheres on regulating MSCs. A) The expression of FAK and p-FAK detected by Western blot analysis. B) Quantitative analysis of the Western blot results. C) The expression of the integrin-α5 gene. D) The expression of the integrin-α8 gene. E) The expression of the Lamb-2 gene. F) The expression of the Spp-1 gene. G) The expression of the Vav-3 gene. H) The expression of the CDC-42 gene. I) Detection of the CDC-42 active protein by a GLISA kit. J) Detection of Rac-1 active protein by a GLISA kit. K) Detection of Rho A active protein by a GLISA kit. L) Mechanism of OBNC hydrogels triggering the membrane receptor switch and activating integrin receptors. (ns: non-significant, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001).

Journal: Bioactive Materials

Article Title: Mechanically sensitized hydrogel microspheres trigger membrane receptor switch for cartilage repair

doi: 10.1016/j.bioactmat.2026.03.017

Figure Lengend Snippet: Effect of OBNC microspheres on regulating MSCs. A) The expression of FAK and p-FAK detected by Western blot analysis. B) Quantitative analysis of the Western blot results. C) The expression of the integrin-α5 gene. D) The expression of the integrin-α8 gene. E) The expression of the Lamb-2 gene. F) The expression of the Spp-1 gene. G) The expression of the Vav-3 gene. H) The expression of the CDC-42 gene. I) Detection of the CDC-42 active protein by a GLISA kit. J) Detection of Rac-1 active protein by a GLISA kit. K) Detection of Rho A active protein by a GLISA kit. L) Mechanism of OBNC hydrogels triggering the membrane receptor switch and activating integrin receptors. (ns: non-significant, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001).

Article Snippet: RhoA GLISA Activation Assay (No. BK124, Cytoskeleton, USA), CDC42 GLISA Activation Assay (No. BK127, Cytoskeleton, USA), and Rac1 GLISA Activation Assay (No. BK128, Cytoskeleton, USA) were used to measure the activation of the GTPase protein family.

Techniques: Expressing, Western Blot, Membrane

hASCs' relative metabolic activity in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining cells' nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Redefining 3D cell culture: Human methacryloyl platelet lysates hydrogels for reliable, consistent, and ethical applications

doi: 10.1016/j.mtbio.2026.103443

Figure Lengend Snippet: hASCs' relative metabolic activity in hPLMA and Matrigel hydrogels for five time points, assessed through the CCK-8 assay. Results are expressed as mean (SD) with n = 5 ( A. ) Representative fluorescence images of hASCs encapsulated in hPLMA and Matrigel hydrogels. The DAPI/Ki-67 staining was performed at 0, 3, and 7 days of culture, with Ki-67 expression (red) and DAPI staining cells' nucleus (blue). The white arrow points to an example of Ki-67 stained cells. Scale bar: 200 μm. ( B. ) Fold change in Ki-67 expression calculated from the CTCF equation using the fluorescence images of Ki-67 expression cultured for 3 and 7 days. The fold change is here compared to day 0. ( C. ) Results are expressed as mean (SD) with n = 3. ∗ p-value <0.05, ∗∗ p-value <0.01, ∗∗∗ p-value <0.001 and ∗∗∗∗ p-value <0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Biological Characterization – Cell Metabolic Activity Quantification: The Cell Counting Kit-8 assay (CCK-8, MCE®, USA) was applied to quantify hASCs' metabolic activity.

Techniques: Activity Assay, CCK-8 Assay, Fluorescence, Staining, Expressing, Cell Culture